ABSTRACT
OBJECTIVE@#To investigate the repairing effect of low intensity pulsed ultrasound (LIPUS) on the Beagle canines periodontal bone defect.@*METHODS@#A total of 12 Beagle dogs with periodontal bone defect model were randomly divided into control group, LIPUS group, guided tissue regeneration (GTR) group and LIPUS+GTR group, with three in each. After completion of the models, no other proceeding was performed in control group; LIPUS group adopt direct exposure to radiation line LIPUS processing 1 week after modeling; GTR group adopted treatment with GTR, following the CTR standard operation reference; LIPUS+GTR group was treated with LIPUS joint GTR. Temperature change before treatment and histopathological change of periodontal tissue after repair was observed.@*RESULTS@#There was no significant difference in temperature changes of periodontal tissue between groups (P>0.05). The amount and maturity of LIPUS+GTR group were superior to other groups; new cementum, dental periodontal bones of GTR group were superior to the control group but less than LIPUS group; new collagen and maturity of the control group is not high relatively.@*CONCLUSIONS@#LIPUS can accelerate the calcium salt deposition and new bone maturation, thus it can serve as promoting periodontal tissue repair, and shortening the periodontal tissue repair time.
Subject(s)
Animals , Dogs , Male , Bone Regeneration , Radiation Effects , Guided Tissue Regeneration, Periodontal , Methods , Histocytochemistry , Jaw , Diagnostic Imaging , Pathology , Radiation Effects , Orthognathic Surgical Procedures , Periodontium , Diagnostic Imaging , Pathology , Radiation Effects , General Surgery , Random Allocation , Sound , Ultrasonic Therapy , Methods , UltrasonographyABSTRACT
<p><b>OBJECTIVE</b>To investigate the expressions of survivin and GRIM-19 in prostatic cancer tissue and their clinical implications.</p><p><b>METHODS</b>We detected the expressions of survivin and GRIM-19 in the tissues of normal prostate (NP), benign prostate hyperplasia (BPH) and prostate cancer (PCa) using immunohistochemical staining, RT-PCR and Western blot, and processed the data by SPSS12.</p><p><b>RESULTS</b>The positive rates of survivin expression were 6.25% , 18.18% and 90.62% in NP, BPH and PCa (P < 0.01), while those of GRIM-19 were 87.50%, 81.82% and 9.37% , respectively (P < 0.01). Semiquantitative RT-PCR and immunohistochemical staining showed that both survivin mRNA and survivin expressions were highly positive in PCa but negative in NP and BPH. Western blot exhibited that the survivin protein was expressed strongly in PCa but weakly in NP and BPH, while the GRIM-19 protein was expressed just contrariwise (P < 0.01).</p><p><b>CONCLUSION</b>The expressions of survivin and GRIM-19 may be closely correlated with the pathogenesis of prostate cancer.</p>